Methyl-seq
Decode the epigenetic landscape with high-resolution methylation insights
What is Methylation Sequencing ?
Methylation sequencing (Methyl-seq) is an approach used to quantify DNA methylation, a key epigenetic modification that regulates gene expression without altering the underlying DNA sequence. In eukaryotic genomes, DNA methylation most commonly occurs at cytosine residues to form 5-methylcytosine (5-mC), particularly within CpG dinucleotides. These methylation patterns play an essential role in embryonic development, cellular differentiation, tissue regeneration, and disease pathogenesis.
Methyl-seq methods enable genome-wide or targeted detection through either chemical conversion or enzymatic approaches. We offer a variety of methyl-seq strategies for genome-wide and targeted insights.
Methyl-seq Solutions at Admera
Whole genome bisulfite sequencing (WGBS)
Whole genome bisulfite sequencing (WGBS) is the gold-standard method for profiling the DNA methylome at single-nucleotide resolution, enabling the study of gene regulation, aberrant methylation, and embryonic development.
Enzymatic methyl-seq (EM-seq)
EM-seq offers an alternative method to WGBS, directly detecting methylation patterns without chemical conversion, generating more accurate and unbiased data with higher coverage and less GC content bias.
Reduced representation bisulfite sequencing (RRBS)
RRBS is a more cost-efficient alternative to WGBS, using methylation-sensitive restriction enzymes to limit sequencing to approximately 20% of the genome with high CpG content.
Targeted Methyl-seq
Targeted methyl-seq uses hybridization probes to capture and enrich biologically relevant methylated regions withing the genome. The targeted nature of this approach reduces sequencing costs and enhances overall coverage depth.
Frequently Asked Questions
Sending samples? See our DNA sample submission guidelines for best practices on sample preparation, packaging, and shipment for the highest quality results.
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Unlike WGS, Methyl-Seq specifically detects methylated cytosines, focusing on epigenetic modifications rather than genetic variation.
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If your sample does not meet our quality control standards at any point in the workflow, we will contact you immediately. We will discuss the specific failure point and provide options, which may include re-submitting the sample, proceeding with a modified workflow, or canceling the project. We believe in transparency and working with you to achieve the best possible outcome.
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We typically work with FFPE, blood, gDNA, cell-free DNA, tissue, and cells. Please contact us if you have further questions about the sample submission requirements.
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Yes. We specialize in handling challenging and low-input samples, including FFPE, blood, fresh frozen tissue, and cell pellets. Our optimized workflows for FFPE samples include a high-yield extraction protocol with improved proteinase K digestion and DNase treatment to minimize DNA and rRNA contamination. This ensures a high success rate and provides the necessary input quality for robust downstream analysis.
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For methyl-seq bioinformatics services, we offer Preliminary QC, trimming, mapping & methylation calling, bisulfite conversion efficiency, differential methylation analysis, (identifying DMR/DMS), annotation, clustering pathway analysis (GO/KEGG) and visualization.
We create customized solutions tailored to your project. Contact us to inquire about a custom bioinformatic solution.
Get the Most out of Your Study
Maximize your project’s potential with advanced analysis solutions. Our team of expert bioinformaticians curate pipelines tailored to your project’s experimental design.
See Methyl-seq in Action
Admera Health provides comprehensive support for all projects, and delivers publication-ready data. Discover how researchers are using Admera Health to advance their studies.