Iso-seq
Complete isoform resolution for the most accurate view of the transcriptome
PacBio Iso-seq is a long-read, full-length transcript sequencing approach that captures PA complete isoforms without assembly, enabling precise characterization of splicing, transcript structure, and gene regulation in eukaryotic systems. Iso-seq sequences full-length cDNA molecules to capture complete transcript isoforms, offering a detailed view of gene expression providing:
Full-length transcript coverage from 5’ to 3’ ends
HiFi accuracy (>99.9%) for confident isoform identification
Robust detection of alternative splicing and novel isoforms
Accurate isoform-level quantification and annotation refinement
What is Iso-Seq?
Applications of Iso-seq
Alternative splicing charcterization
Comprehensive discovery and focused panels available for whole genome insights or specific genomic regions
Novel isoform discovery and transcriptome annotation
Flexible workflows and panels can be designed by our scientists to meet the requirements of your project
Fusion transcript detection in oncology
Solutions for fresh, frozen, FFPE, from any genome
Advanced data interpretation enable actionable insights quickly
Allele-specific and phased transcript
Iso-seq Workflow
Every Iso-seq project is handled with meticulous attention to detail. Our workflow includes rigorous quality control at the sample, library, sequencing, and data levels, ensuring accurate, high-quality results. From cDNA preparation to full-length transcript analysis, we prioritize reliability and reproducibility to deliver biologically meaningful insights for your research.
Frequently Asked Questions
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We accept eukaryotic samples and recommend starting with total RNA:
Total RNA 1–2 µg, minimum 500 ng per sample RIN ≥ 7
OD260/280: 1.8–2.0; OD260/230: 2.0–2.2
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We provide comprehensive downstream analysis and interpretation including:
• Differential expression (DE) at gene and isoform level• GSEA (Gene Set Enrichment Analysis)
• GO (Gene Ontology) enrichment analysis
• KEGG pathway analysis
• Splicing variant analysis
If you have further questions, please contact us to get in touch with a specialist.
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Our typical turnaround time for Iso-seq projects is 4-6 weeks. Please connect with us if you require expedited services, we are happy to create a timeline that works best for your project goals.
More PacBio Long-Read Services
Full-length 16s Amplicon-seq
With PacBio Revio, full-length transcript sequencing becomes seamless. HiFi reads span entire isoforms end to end, eliminating the need for assembly and enabling confident detection of alternative splicing, fusion events, and novel transcripts. This level of clarity supports functional annotation, biomarker discovery, and both bulk and single-cell transcriptomics.
Full-Length WGS
Revio delivers highly accurate long reads that resolve complex genomic regions and structural variants that short-read platforms often miss. HiFi WGS supports de novo assembly, haplotype phasing, variant calling, and comprehensive analysis across human, model organism, and microbial genomes.
AAV Sequencing
Targeted sequencing with Revio allows long-range amplicons to be captured in a single read, covering full genes or difficult regions with ease. This approach is ideal for applications such as CRISPR edit verification, microbial typing, oncology panels, HLA sequencing, and low-frequency variant detection.
PacBio PureTarget
Revio’s long reads improve taxonomic resolution and strain-level identification in complex microbial communities. HiFi data enables accurate assembly, functional profiling, and detection of low-abundance organisms without the bias introduced by short-read assembly.