Amplicon-seq
Amplify specific or targeted regions of interest
What is Amplicon-seq and what is it used for?
Amplicon sequencing is a highly targeted method for studying genetic variation in specific regions of the genome. The amplicons are generated by PCR, pooled, and sequenced. The sequencing data provides researchers targeted insights with low-level inputs. This approach is particularly useful for applications like identifying specific mutations, analyzing highly variable regions, and detecting rare variants within a complex sample.
Admera Health provides end-to-end amplicon-seq services from project consultation and sample QC to library preparation, sequencing, and bioinformatic analysis. Whether you’re profiling microbial communities, assessing genetic variants, or evaluating CRISPR edits, our validated workflows help ensure reproducible, ready-to-use results.
Amplicon-seq Methods
CRISPR screening, editing validation, and INDEL calling
In a CRISPR screen, amplicon-based sequencing of the guide regions reveals which gRNAs are enriched or depleted. Following the screen, this approach enables quantification of on-target edits and identify off-target events at single-base resolution. Ideal for validation of gene editing experiments, screening of edited clones, or functional studies.
16S rRNA gene sequencing
Target hypervariable regions of the bacterial 16S rRNA gene. Ideal for microbial community profiling in complex or heterogeneous samples.
Custom Amplicons
Our team can design custom primers for locus of interest for high sensitivity and coverage of variant detection.
Long-read Amplicon-seq
Targeted sequencing with HiFi reads allows you to sequence only the genomic regions you care about at scale. The PacBio amplicon sequencing workflow is designed to generate high-accuracy, long-read sequences of targeted genomic regions, ideal for applications like microbial profiling, HLA typing, and variant detection (from single nucleotide variants and indels, to structural variants).
Applications of Amplicon Sequencing
Microbiome and Metagenomic Studies
Targeted 16s rRNA gene sequencing enables precise bacterial profiling in human, animal, and environmental samples.
Functional Gene Analysis
Assess specific genes of interest, including antimicrobial resistance (AMR) markers, metabolic pathways, or virulence factors.
CRISPR Gene Editing
and Validation
Evaluate on-target and off-target editing outcomes at specific loci with high precision.
Detect SNPs, insertions/deletions, and rare alleles
Genetic Variant Analysis
Get the Most out of Your Study
Maximize your project’s potential with advanced analysis solutions. Our team of expert bioinformaticians curate pipelines tailored to your project’s experimental design.
See Amplicon-seq in Action
Admera Health provides comprehensive support for all projects, and delivers publication-ready data. Discover how researchers are using Admera Health to advance their studies.