Long-Read Sequencing


Discover complete genomic insights with high-fidelity, long-read sequencing powered by PacBio Revio

4-5 weeks

Turnaround for Library Preparation & Sequencing

US Operations

100%

CERTIFIED PROVIDER

Annual Recertifications

Long-Read Sequencing Designed for Complex Questions

PacBio Flyers - WGS, Isoseq, General - transparent
PacBio Flyers - WGS, Isoseq, General - transparent

Long-read sequencing is a next-gen sequencing (NGS) approach that produces reads thousands to tens of thousands of bases long. The added context helps enable more confident detection of structural variants, accurate analysis of complex or repetitive regions, improved haplotype phasing, and higher-contiguity assemblies.

Admera Health recommend long-reads sequencing when your project depends on:

  • Structural variant detection and complex rearrangements

  • Haplotype phasing

  • Analysis of repeat-rich or hard-to-map regions

  • De novo genome assembly and reference improvement

  • Full-length transcript resolution and isoform discovery

  • Native methylation profiling

Advantages of long-read sequencing often include improved resolution of complex genomic regions, more complete assemblies, and clearer interpretation of phased variants and full-length transcripts.

PacBio Certified Service Provider

As a PacBio Certified Service Provider, Admera Health is part of a global network of validated sequencing labs offering access to HiFi sequencing. We deliver long-read sequencing services designed to resolve complex variation, repetitive regions, and full-length biology with a clarity short reads often can't match. Our services run on the PacBio Revio system powered by SPRQ-Nx chemistry, delivering high-throughput HiFi sequencing at Q30+ base accuracy through the PacBio SMRT sequencing workflow and Revio SMRT cell format. Every standard run also includes on-instrument methylation calling (5mC, 5hmC, and 6mA) alongside sequence data, with no extra prep step required.

We support DNA, RNA, and genome sequencing applications across research and translational programs, backed by sample QC, library prep, and optional bioinformatics support.

Want to learn more about the PacBio platform behind our sequencing services? Check out the flyer for a technology overview.

Why Scientists Choose Admera Health for Long-Read Sequencing

As a PacBio Certified Service Provider, Admera Health is part of a global network of validated labs offering access to HiFi sequencing backed by turnaround like no other.

HiFi accuracy at scale

Revio SPRQ-Nx chemistry delivers Q30+ base accuracy with long read lengths, from small studies to large cohorts.

Expert support

Admera Health manages sample QC, library preparation, sequencing, and delivery of analysis-ready data.

Flexible project scale

From targeted panels and amplicons to whole genomes and population studies.

Long-Read vs. Short-Read Sequencing

Know When to Use Each

Short read sequencing remains an efficient, cost-effective choice for many high-throughput applications (for example, standard variant detection and RNA profiling). Long-read sequencing provides highly accurate, contiguous reads that can capture complex genomic regions, structural variants, and full-length transcripts.

Many teams work with Admera Health to combine both approaches using long-reads to resolve structure and short reads to add depth based on the biological question, turnaround, and budget. In many next-gen sequencing pipelines, short read sequencing is used for large-scale screening and long-read sequencing is used to resolve complex loci and structural variation.

table comparison chart of long-read vs short-read general

Overview

table comparison chart of long-read vs short-read applications

By Application

Have more questions?

Long-Read Services at Admera

Long-read RNA Sequencing

Full-length transcript sequencing becomes seamless. HiFi reads span entire isoforms end to end, eliminating the need for assembly and enabling confident detection of alternative splicing, fusion events, and novel transcripts. This level of clarity supports functional annotation, biomarker discovery, and both bulk and single-cell transcriptomics.

Whole Genome Sequencing

Revio delivers highly accurate long reads that resolve complex genomic regions and structural variants that short-read platforms often miss. HiFi WGS supports de novo assembly, haplotype phasing, variant calling, and comprehensive analysis across human, model organism, and microbial genomes.

Amplicon Sequencing (Amplicon-seq)

Targeted sequencing with Revio allows long-range amplicons to be captured in a single read, covering full genes or difficult regions with ease. This approach is ideal for applications such as CRISPR edit verification, microbial typing, oncology panels, HLA sequencing, and low-frequency variant detection.

Metagenomics

Revio’s long reads improve taxonomic resolution and strain-level identification in complex microbial communities. HiFi data enables accurate assembly, functional profiling, and detection of low-abundance organisms without the bias introduced by short-read assembly.

Bioinformatics Services for Long-Read Sequencing Data

For teams who want end-to-end support, Admera Health offers bioinformatics services for long-read sequencing projects, which can include QC summaries, assembly and evaluation, structural variant calling, phasing, transcript isoform analysis, and tailored reporting aligned to your deliverables.

Every deliverable is aligned to your research goals from raw QC through final analysis.

Save on Long-Reads with Revio

Access savings on long-read savings on RNA, DNA, and premade library sequencing when you run on the Revio.