High Throughput

RNA-seq

Accelerating transcriptome research, faster and smarter

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RNA-seq is a transformative method for exploring the effects of drugs on gene expression across the entire transcriptome. However, conventional assays can be both expensive and labor-intensive. Several platforms leverage RNA barcoding and multiplexing RNA-seq to provide rapid, cost-effective, and high-throughput transcriptome analysis, significantly enhancing the statistical power of differential gene expression studies.

These low-cost, scalable for high throughput and automation compatible approaches can drastically accelerate transcriptome research for drug discovery and other large-scale comparative studies.

What is High-Throughput RNA-seq?

Discover how researchers are using Admera Health to advance their Transcriptomics studies.

Transcriptomics In Action

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Key Features & Highlights

Flexible starting requirements

Cells or RNA from low starting input material

Rapid turnaround time

Data delivery within 10 business days

Advanced multiplexing

Increased multiplexing capabilities result in lower sequencing costs

Cutting-edge technology

3’ Tag-seq technology results in high-throughput data with fewer sequencing reads at reduced costs

Research Applications

Drug Discovery Mechanisms
of Action

Uncover potential drug targets, validate therapeutic efficacy, and reveal mechanisms of action

Gene Expression Profiling

Detect changes in gene expression patterns, compare molecular signatures at scale, and generate robust datasets for biomarker discovery & therapeutic developments

Unlock gene function and pathway insights at scale with high-throughput RNA-seq for functional genomics

Functional Genomics

Prioritize active compounds, elucidate potential mechanisms of action, and accelerate drug discovery

Compound Screening

Drive precision care with high-throughput RNA-seq, uncovering patient-specific expression profiles to inform targeted therapies

Personalized Medicine

Model diseases at the molecular level with high-throughput RNA-seq, uncovering pathways, biomarkers, and therapeutic insights at scale

Disease Modeling

Multiple High-Throughput RNA-seq Platforms Available

Table 1. Comparison table of various high-throughput RNA-seq platforms and features for each workflow. Key features include input type, input range per well, and maximum sample pooling capabilities.

*For highly expressed gene while deep sequencing enables detection of low-abundance transcripts.

Our Optimized Workflow

Our workflow is designed to accommodate various techniques, including Smart-seq 3', QuantSeq Pool, DRUG-Seq, and BRB-Seq. The process begins with sample barcoding in plate format to enable multiplexing followed by sample pooling. Pooled samples undergo library preparation and sequencing followed by data analysis to extract valuable insights into gene expression patterns.

Frequently Asked Questions

Sending samples? See our RNA sample submission guidelines for best practices on sample preparation, packaging, and shipment for the highest quality results.

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We accept a wide variety of sample types, including FFPE, fresh frozen tissue, blood, cell pellets, and purified RNA. For purified RNA, we require a minimum concentration of 50 ng/µL in a total volume of 50 µL. We recommend providing a higher input amount for low-abundance transcripts. Please submit a completed sample submission form with your shipment.
See Sample Submission Guidelines here.
If your sample does not meet our quality control standards at any point in the workflow, we will contact you immediately. We will discuss the specific failure point and provide options, which may include re-submitting the sample, proceeding with a modified workflow, or canceling the project. We believe in transparency and working with you to achieve the best possible outcome.
Our standard turnaround time is typically 10-15 business days, though this can vary depending on the sample type, project complexity, and required sequencing depth. Expedited services are available upon request. Please contact us for a detailed quote and timeline specific to your project.
Our workflows are designed to maximize data quality from start to finish. We use specialized protocols for challenging samples like FFPE to ensure high-quality RNA extraction. During library preparation, we use Unique Dual Indexes (UDIs) to minimize index hopping and Unique Molecular Identifiers (UMIs) to correct for PCR duplicates, ensuring accurate quantification. We also perform stringent quality control checks at every step to guarantee reliable and usable data.
Yes. We specialize in handling challenging and low-input samples, including FFPE, blood, fresh frozen tissue, and cell pellets. Our optimized workflows for FFPE samples include a high-yield extraction protocol with improved proteinase K digestion and DNase treatment to minimize DNA and rRNA contamination. This ensures a high success rate and provides the necessary input quality for robust downstream analysis.
Our bioinformatics services are comprehensive and customizable. For Bulk RNA-seq, we provide trimming and QC, mapping, counting, normalization, and differential expression analysis (DE). For projects involving non-model organisms, we offer de novo transcriptome assembly. Additional analysis options include alternative splicing detection, gene fusion analysis, and functional enrichment analysis (MA-plot, PCA, heatmaps, volcano plots, and GO enrichment).

Maximize your project’s potential with advanced analysis solutions. Our team of expert bioinformaticians curate pipelines tailored to your project’s experimental design.